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KMID : 1144820210270040216
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대한의생명과학회지
2021 Volume.27 No. 4 p.216 ~ p.222
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Expression of miR-29a in whole Blood of Patients with Colorectal Neoplasm
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Hwang Da-Som
Kim Da-Hye
Chang Yun-Hee
Hirgo Workneh Korma
Lee Hye-Young
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Abstract
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Colorectal cancer (CRC) is major cancer with high incidence and mortality worldwide. It is known that most CRCs arise from precursor adenomatous polyps (APs). Recently, microRNA (miRNA) has been proposed as a biomarker for various cancers including CRC. In this study, the expression patterns of miR-29a in the whole blood (WB) of CRC, AP, and control groups were analyzed by reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) to evaluate the expression level of miR-29a in patients with colorectal neoplasm (CRN) including CRC and AP. As a result, the relative expression of miR-29a was significantly decreased in the patients with CRN compared to the control group (P<0.001). The results were in agreement with previous in vitro cell studies and studies that used tissue and feces samples, suggesting that miR-29a in WB may be useful in demonstrating the status of colorectal tissue. Additionally, we divided the control group into healthy control (HC) without any colorectal symptoms and non-tumor control (NTC) with colorectal symptoms but without any CRN. And then the relative expression of miR-29a was also significantly decreased in the NTC group compared to the HC group (P<0.001). Therefore, our study revealed that miR-29a can differentiate patients with CRN from HC group, but they are also involved in the early stage of inflammatory response and cannot be specific biomarkers for CRN.
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KEYWORD
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Colorectal cancer, Adenomatous polyp, Colorectal neoplasm, MicroRNA, MicroRNA-29a, Whole blood, Biomarker
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